When a certain protein or enzyme inside the cell binds to a small molecule inhibitor, can it be detected by western blot? Can the antibody still bind to it?
Western blot detects the presence and expression levels of proteins by using specific antibodies that bind to the target protein. When a protein or enzyme within cells binds to a small molecule inhibitor, Western blot can detect this. The specific antibodies can still bind to other regions of the protein to form an antigen-antibody complex, which can be detected through chemiluminescence or staining methods. These methods can produce visual signals to determine the presence and expression level of the target protein.
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