Label Transfer Method Protein Interaction Analysis

The tag transfer method combined with cross-linking is used to study protein-protein interactions by tagging proteins that interact with the target protein. This method can be used to discover new interactions, validate interactions detected by other techniques, and it can be applied to the study of protein complexes. Additionally, the tag transfer method can detect weak and transient protein interactions that are often undetectable by co-immunoprecipitation or Pull-Down methods.

In a typical tag transfer assay, the bait protein first reacts with the reactive part of a radiolabeled, fluorescent, or biotin-labeled tag transfer reagent (LTR). The tagged bait protein is then allowed to interact with the target protein in vitro to form a stable or temporary complex, and is exposed to UV light to activate the photosensitive group on the cross-linking agent. Subsequently, it covalently binds to the prey protein. Finally, by cleaving the cross-linker spacer arm to release the attached bait protein, the tag transfer is completed.

Tag transfer method for protein-protein interaction

Fluorescent or radiolabeled aryl azides, carbenes, or benzophenones linked to hormones, peptides, or nucleic acids can be used to label and explore previously unknown target proteins. The tag transfer method has been widely used in nucleic acid-protein interaction examination. Using the latest tag transfer strategies, Biotech Pekin can select the appropriate cross-linker according to your experimental goals and conduct subsequent analyses to assist you in exploring target protein-protein interactions.

Biotech Pekin offers a variety of strategies for protein-protein interaction analysis

Protein-protein interaction analysis
Mass spectrometry analysis of protein-protein interactions
Protein interaction analysis using co-immunoprecipitation (CO-IP) combined with mass spectrometry
Protein interaction analysis using GST fusion protein Pull-down technology combined with mass spectrometry
Protein interaction analysis using SILAC combined with co-immunoprecipitation and mass spectrometry
Cross-linking method for protein-protein interaction analysis
Far-Western Blot analysis

One-stop service for tag transfer method protein interaction analysis

You only need to place an order and send your samples

Biotech Pekin one-stop service includes: sample processing - machine analysis - data analysis - project report