Monosaccharide Composition Analysis by ATR FT-IR Method
The ATR FT-IR method (Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy) is an effective technique for analyzing the composition of monosaccharides. This technique utilizes the infrared absorption characteristics of specific functional groups within monosaccharide molecules to identify and quantify them.
Basic Principle:
In ATR FT-IR analysis, infrared light passes through the sample, with some of the light being absorbed by the molecules in the sample and some being reflected. Molecules with different chemical structures will absorb light at specific infrared wavelengths, and these absorption peaks can be used to identify specific compounds in the sample.
Monosaccharides such as glucose and fructose contain specific functional groups like hydroxyl (OH) and carbonyl (C=O), which have characteristic absorptions at specific infrared bands. By analyzing these absorption peaks, the type and content of monosaccharides in the sample can be determined.
Figure 1. Basic Principle of ATR FT-IR
Technical Advantages:
The ATR FT-IR method has advantages such as being fast, requiring minimal sample preparation, and being non-destructive. It can be used for rapid screening and analysis, making it particularly suitable for quick analysis of multiple or complex samples.
This method is not only suitable for analyzing monosaccharides but is also commonly used for polysaccharides, glycoproteins, and other sugar-containing biomolecules. It has wide applications in food science, biotechnology, and the pharmaceutical industry.
Limitations:
Although the ATR FT-IR method is highly effective in monosaccharide analysis, it has some limitations. For example, monosaccharides with similar structures may have very similar infrared absorption peaks, which may require the combination of other analytical methods for accurate identification.
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