Vibrational Circular Dichroism (VCD) Sample Testing
Vibrational Circular Dichroism (VCD) is a scientifically effective experimental method used to study the structure and dynamics of chiral molecules. VCD can provide richer information compared to traditional Infrared Spectroscopy (IR) or Raman Spectroscopy, enabling researchers to gain a deeper understanding of the sample's structure.
1. Sample Preparation
The purity and quality of the sample have a significant impact on the quality of VCD results. Therefore, it is first necessary to ensure that the sample is of sufficiently high purity and free from impurities or other contaminants. The sample usually needs to be tested in solution form, so selecting an appropriate solvent is also very important. It must be ensured that the solvent does not interfere with the VCD test or that its effects can be eliminated through subsequent data processing steps.
2. VCD Testing
VCD testing is usually carried out by professional analytical laboratories. The sample is tested along a specific light path at a specific temperature. By changing the polarization state of the light, the difference in absorption of the sample to different polarized light can be measured. This data can be used to calculate the VCD spectrum of the sample.
3. Data Processing and Analysis
The analysis of VCD spectra requires specialized knowledge and skills. First, background noise and other possible interference factors need to be eliminated. Then, specialized software is used to deconvolute the spectral lines to obtain the intensity and frequency of each vibrational mode. By comparing with theoretical models, the structure and dynamic information of the sample can be inferred.
BiotechPack, A Biopharmaceutical Characterization and Multi-Omics Mass Spectrometry (MS) Services Provider
Related Services:
Protein Circular Dichroism Analysis
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