How much gram of lychee peel is enough for transcriptome sequencing?
The amount of sample required for transcriptome sequencing mainly depends on the RNA content and extraction efficiency of the sample itself. For plant tissues (such as lychee peel), it is usually recommended:
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Prepare 0.5–1 g of fresh tissue per sample;
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If the sample has high water content or low RNA content, it may be increased appropriately to 1–2 g;
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If the sample is provided as pre-ground powder in liquid nitrogen, about 0.5 g is generally sufficient, provided the grinding is uniform and there is no degradation.
It is recommended to snap-freeze in liquid nitrogen as soon as possible, grind thoroughly, and avoid RNA degradation. Samples can be divided for frozen storage and transported on dry ice or in liquid nitrogen. If planning multiple replicates or time points, weigh and preserve each sample independently to avoid mixing.
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