Can all proteins pulled down be identified by mass spectrometry?
Not all proteins pulled down in a pull-down assay can be identified by mass spectrometry. The success of identification depends on several factors:
1. Protein abundance:
High-abundance proteins are easier to identify, whereas low-abundance proteins may be difficult to detect.
2. Sample preparation:
Proteins may be lost during sample processing, especially those that are prone to degradation or adhere to the walls of the tube.
3. Mass spectrometry sensitivity:
The sensitivity of the mass spectrometer determines the minimum abundance of proteins that can be detected.
If a low-abundance protein is captured in a pull-down experiment and some of it is lost during sample processing, it may still be difficult to detect with even high-sensitivity mass spectrometry equipment, especially in complex samples like cell lysates.
BiotechPack, A Biopharmaceutical Characterization and Multi-Omics Mass Spectrometry (MS) Services Provider
Related Services:
GST pull-down protein interaction analysis
Mass spectrometry analysis of protein interactions
Pull-down target protein mass spectrometry identification
SILAC and co-immunoprecipitation coupled mass spectrometry for protein interaction analysis
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