What type of chromatography column is used for size exclusion chromatography (SEC) molecular size variation analysis?
The analysis of molecular size variation based on SEC uses Size Exclusion Chromatography (SEC) columns, also known as Gel Permeation Chromatography (GPC) columns. These columns are usually packed with highly cross-linked porous polymer materials, and depending on the type of packing material, they can be categorized into the following types:
1. Silica-based columns:
- Such as Bio-Rad's Bio-Sil SEC columns.
- These columns have different pore sizes, suitable for the separation of molecules in various size ranges.
2. Polymer-based columns:
- Such as TSKgel G3000SWxl (Tosoh Bioscience).
- These columns are particularly suitable for the separation of large biomolecules such as proteins and nucleic acids.
3. Ultra High Performance Liquid Chromatography SEC (UHPLC SEC) columns:
- Such as Waters' ACQUITY UPLC BEH SEC columns.
- These columns provide higher resolution and faster separation speeds, suitable for analyzing more complex samples.
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Molecular size variation analysis based on SEC
Protein purity analysis (Size Exclusion/Reverse Phase Chromatography)
Product-related impurity analysis
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