Protein Acetylation Detection

Protein acetylation detection includes the detection of whether proteins have undergone acetylation modifications, identifying the sites of acetylation, and quantifying the acetylated proteins. Biotech company Bertie Parker provides mass spectrometry-based services.Protein Acetylation DetectionService.

Protein Acetylation

Protein acetylation is one of the major post-translational modifications (PTMs) in eukaryotes, where an acetyl group from a compound is transferred to a specific site on the polypeptide chain through enzymatic action. Protein acetylation typically occurs in two different forms: one is N-terminal acetylation, which is one of the most common co-translational covalent modifications in eukaryotes, playing a crucial role in the regulation and function of various proteins, such as their synthesis, stability, and localization. Approximately 85% of human proteins and 68% of yeast proteins undergo Nα-terminal acetylation. The other form is acetylation on lysine residues, which depends on acetyl-CoA as an acetyl donor. In histone acetylation and deacetylation, as part of gene regulation, histones are acetylated and deacetylated on lysine residues in their N-terminal tails. Typically, histone acetylation and deacetylation are catalyzed by lysine acetyltransferases (KATs) or lysine deacetylases (KDACs), and KATs and KDACs can also alter the acetylation state of non-histone proteins.

Protein Acetylation Detection

Protein Acetylation Detection

Protein acetylation detection mainly includes the identification of protein acetylation and the quantification of acetylated proteins. Current methods for analyzing protein acetylation modifications primarily include mass spectrometry-based analysis and western blot-based analysis. Mass spectrometry does not require specific antibodies against acetylation when identifying acetylated proteins, whereas western blot requires acetylation antibodies.

Related Services:

Acetylation Quantitative Proteomics Research
Post-translational Modification Proteomics Analysis