Acetylation Modified Proteomics Analysis

Acetylation modification proteomics analysis refers to the study of protein acetylation modifications at the proteomics level, including the identification and quantification of acetylated proteins. Biotech company Biotyper provides mass spectrometry-based services.Acetylated proteome researchservices.

Acetylation modification proteomics analysis

Protein acetylation includes histone acetylation and non-histone acetylation. Histone acetylation mainly involves lysine acetylation and has been extensively studied in gene transcription regulation. Non-histone acetylation constitutes the major portion of acetylated proteins in mammalian cells and participates in all major biological processes, including protein localization, transfer, function, and degradation, as well as transcription and replication of genetic material. Besides significant biological functions, acetylated proteins and their regulatory enzymes are closely associated with aging and various diseases such as cancer, neurodegenerative disorders, and cardiovascular diseases. Therefore, studying the acetylation modification proteome helps further explore cellular life activities and understand the pathogenesis of related diseases.

Mass spectrometry analysis of the acetylated proteome

Mass spectrometry analysis technology can identify, locate acetylation sites, and quantify acetylated proteins in the proteome. When mass spectrometry is used in acetylation quantitative proteome research, due to the low abundance and wide dynamic range of acetylated proteins in biological samples, it is necessary to enrich acetylated peptides first, and then perform quantitative analysis on the enriched acetylated peptide samples. Mass spectrometry quantitation of acetylated proteomes uses peptide pre-separation to reduce the impact of high-abundance proteins, combined with immunoprecipitation to efficiently enrich acetylated peptides through antibodies, thus achieving large-scale identification and quantification of acetylation. To identify and quantify more acetylated peptides, various enzymes can be used for protein sample digestion during the enzymatic digestion process.

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Acetylation quantitative proteome research

Acetylation modification proteomics analysis