Protein Phosphorylation Level Detection

Protein phosphorylation level detection includes the quantification of multiple or individual phosphorylated proteins or the assessment of the phosphorylation level of target proteins. BGI Tech provides mass spectrometry-based protein phosphorylation level detection services.

Protein phosphorylation detection

Protein kinases transfer phosphate groups from ATP to protein substrates, directly affecting the activity and function of target proteins. Protein phosphorylation regulates a wide range of cellular activities, including the cell cycle, differentiation, metabolism, and more. Additionally, abnormal phosphorylation events are associated with many disease states. Studying protein phosphorylation helps to understand life activities and elucidate the mechanisms of diseases related to abnormal phosphorylation. Common methods to study protein phosphorylation include SDS-PAGE, western blot, flow cytometry, kinase activity assays, enzyme-linked immunosorbent assays (ELISA), antibody arrays, and mass spectrometry. Among these, western blot, ELISA, and mass spectrometry can be used to detect protein phosphorylation levels.

Protein phosphorylation level detection

Protein phosphorylation level detection

Protein phosphorylation level detection refers to the quantitative study of protein phosphorylation, including the quantification of multiple or individual phosphorylated proteins or the assessment of the phosphorylation state of a specific protein. Western blot and mass spectrometry are commonly used for analyzing protein phosphorylation levels. Western blot primarily relies on antibodies to detect the phosphorylation level of target proteins; it is simple to operate and requires basic equipment but is not suitable for large-scale analysis of complex biological samples' phosphorylation levels. Mass spectrometry-based techniques can be used for the simultaneous detection of the phosphorylation levels of multiple target proteins.

Related services

Post-translational modification proteomics analysis
Phosphorylation quantitative proteomics