Untargeted Proteomics

Non-targeted proteomics involves the analysis and study of the entire proteome without specifying particular proteins. Biotech company Biotree provides mass spectrometry-based non-targeted proteomics analysis services.

Non-targeted Proteomics

Compared to targeted proteomics, non-targeted proteomics is a more 'global' analysis that does not focus on any specific protein. In non-targeted proteomics, the questions researchers aim to address might be broader, such as 'What proteins can be found in this sample?' or 'Which proteins show differential expression across different samples?' Therefore, non-targeted proteomics can also be referred to as 'discovery proteomics,' for example, for biomarker discovery. By its nature, non-targeted proteomics is less sensitive than targeted proteomics, but it can measure more proteins. Researchers can discover new proteins using non-targeted proteomics without relying on the literature to determine which proteins are involved in a sample and measure protein expression levels.

Non-targeted Proteomics Analysis Techniques

Common non-targeted proteomics analysis techniques are often related to mass spectrometry and generally involve quantitative techniques since both qualitative and quantitative assessments of the proteome are conducted in quantitative studies. Non-targeted quantitative proteomics techniques include labeled quantification and label-free quantification, with labeled quantification further divided into in vivo and in vitro labeling. A common in vivo labeling method isSILAC-based proteome quantification, which is a live cell labeling quantitative proteomics technique; a common in vitro labeling method isTMT/iTRAQ-based proteome quantification, which can be used for labeled quantification of samples that cannot be passaged. The above labeled quantification techniques and generallabel-free quantificationall belong to DDA (Data-Dependent Acquisition) mass spectrometry techniques.SWATH TechnologyAs a DIA (Data-Independent Acquisition) mass spectrometry technique, it combines the advantages of high throughput and precise quantification, enabling proteome identification and quantification without sample labeling, and is also a non-targeted proteomics analysis technique.

Related Services:

Quantitative Proteomics Analysis based on SILAC/Dimethyl Labeling
TMT/iTRAQ/MultiNotch Quantitative Proteomics Analysis
Label-Free Quantitative Proteomics Analysis
SWATH Quantitative Proteomics Services

Non-targeted Proteomics