PRM Proteomics
PRM proteomics refers to the use of PRM for proteomic analysis, enabling targeted quantification of target proteomes, including both absolute and relative quantification. Biotech company BGI offersPRM quantitative proteomics analysisservices.
Introduction to PRM
PRM (parallel reaction monitoring) is an ion monitoring technique based on high-resolution and high-precision mass spectrometry, used for targeted quantitative proteomics studies of target protein molecules. PRM is currently the mainstream method for targeted proteomics data acquisition, achieving targeted relative or absolute quantification of target proteins/modification peptides by selectively detecting specific peptide segments or target peptide segments (e.g., peptides with post-translational modifications).
Principle of PRM Proteomics
PRM is based on a high-resolution mass spectrometry platform represented by Q-Orbitrap. After samples are ionized in the ion source, PRM first uses the quadrupole Q1 to selectively detect the precursor ions of target peptides, which are then fragmented in the collision cell (Q2). Finally, the Orbitrap or TOF mass analyzer performs high-resolution and high-precision scans of all product ions. It not only has the ability for quantitative analysis of target proteins but also qualitative capabilities. PRM achieves mass accuracy at the ppm level, effectively eliminating background interference and false positives, significantly improving detection limits and sensitivity in complex backgrounds. Full scanning of product ions is conducted without the need to select ion pairs or optimize fragmentation energy, making it easier to develop assay methods.
PRM Proteomics
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