Peptide Synthesis - What is the Fmoc Strategy in SPPS?
SPPS is the synthesis of peptides based on solid-phase support, also known as resin, which is modified with a linker to provide the reactive group for the first amino acid. Peptides are synthesized from another peptide via an amide bond between the amine group and the carboxyl group under pre-activation or in-situ activation conditions. To avoid unwanted reactions, protecting groups are used to temporarily mask the reactive groups on the Nα position and side chains of the amino acids. Fmoc is one of the commonly used Nα-protecting groups, with relatively obvious advantages. The progress of each deprotection reaction can be monitored in real-time by spectrophotometry at 300-320 nm, where the Fmoc group is cleaved and released. After the desired polypeptide is synthesized, since the Fmoc strategy uses acid-labile linkers, the resin-bound polypeptide is deprotected and cleaved from the solid support using TFA.
How to order?
