How to remove impurities such as trifluoroacetic acid (TFA) and acetonitrile from the final product after reverse phase chromatography purification?

As long as it does not exceed the tolerance range, lyophilization may be the simplest and most effective method to remove most of the trifluoroacetic acid (TFA) and acetonitrile. However, this method cannot meet the requirements of some peptide drugs with higher TFA content standards. Special salt conversion and desalination treatments should be performed for them.

• Peptide purity analysis