Protein Tandem Mass Spectrometry Identification
Tandem Mass SpectrometryTechnology(Tandem Mass Spectrometry, MS/MS) is an efficient,precise, sensitivemethodfor proteinsfor qualitative and quantitativeidentification, widely used in fields such as proteomics research, biopharmaceutical quality control, and disease biomarker discovery.Itachievesthe identificationof target proteins, includingthe identification of unknown proteins.This technologyemerged in the early 1990s, initially dominated by liquid chromatography coupled with mass spectrometry (LC-MS/MS). Withthe development ofmatrix-assisted laser desorption/ionization (MALDI) and other technologies, protein mass spectrometry identification entered a new era.Nowadaystandem mass spectrometrytechnologyhas become an important tool in proteomics research, significantly contributing to the analysis of protein structure and function in complex biological samples,helping to reveal protein interactions and signal transduction mechanisms within organisms, advancing biological science and disease treatment.
BaiTaiPikeBiotechnology BTPTandem Mass Spectrometry Protein IdentificationTechnical Process
1.Sample Preparation:
Select the appropriate method (such as high-performance liquid chromatography or gel electrophoresis) to purify, concentrate, and desalt the protein samples provided by the customer, removing impurities and increasing protein purity.
2.Protein Digestion:
Performpurificationof proteins usingmultiple enzyme digestion to cleave proteins into peptide fragments,facilitating subsequent mass spectrometry analysis.
3.Peptide Separation:
Use nano high-performance liquid chromatography (nano-HPLC) to separate peptides, effectively improving resolution and detection sensitivity.
4.TandemMass Spectrometry Analysis:
We use high-resolution, high-sensitivity mass spectrometers(Thermo Fisher's Q ExactiveHF mass spectrometry platform, Orbitrap Fusion mass spectrometry platform, Orbitrap Fusion Lumos, Orbitrap Exploris 240)to perform mass and fragment ion analysis on the separated peptide fragments using tandem mass spectrometry (MS/MS).
5.DataAnalysis:
Useprofessionaldata processing software Mascot and protein databases for peptide mass matching and fragment ion searching, ultimately achieving protein identification, including detailed information such as protein names, amino acid sequences, and matching scores.
Main Applications of Tandem Mass Spectrometry Protein Identification Technology
1. High-throughput Protein Identification:
Tandem mass spectrometry plays a key role in proteomics research, used to identify and quantify proteins in biological samples, revealing protein expression differences, interactions, and functions.
2. Post-translational Modification Studies:
Used to identify post-translational modifications of proteins (such as phosphorylation, acetylation, glycosylation), revealing the impact of modifications on protein functions and signal transduction.
3. Protein Interaction Studies:
By identifying components within protein complexes, it reveals protein interaction relationships, providing a basis for biological signaling pathway research.
4. Protein Structure Analysis:
MS/MS technology can analyze the primary structure (amino acid sequence) and some secondary and tertiary structure information of proteins, providing reference data for protein structure research.
5. Disease Diagnosis and Biomarker Research:
MS/MS technology can be used for the identification and quantification of disease-related proteins, providing a basis for disease diagnosis and prognosis evaluation.
About Sample
|
Sample Type |
Cell Lysate |
Tissue Extract |
Body Fluid Sample |
Gene Expression Product |
Protein Complex |
|
Sample Quantity |
50-200ug |
100-500ug |
10-100ug |
1-10ug |
5-50ug |
Chinese/English Project Report
In the technical report, BaiTaiPike will provide you with a detailed bilingual technical report in both Chinese and English, including:
1. Experimental Steps (Chinese/English)
2. Relevant Mass Spectrometry Parameters (Chinese/English)
3. Detailed Information on Tandem Mass Spectrometry Protein Identification
4. Mass Spectrometry Images
5. Raw Data
One-stop Service for Tandem Mass Spectrometry Protein Identification
You only need to place an order - send samples
BaiTaiPike's one-stop service completes: sample processing - machine analysis - data analysis - project report
Related Services
Protein Mass Spectrometry Identification
Protein Spot, Gel Strip, IP Sample Protein Identification
Pull-down Target Protein Mass Spectrometry Identification
Shotgun Proteomics Protein Identification
Membrane Protein Identification Service
Peptide Mass Spectrometry Identification
Determination of Protein Primary Structure
Protein Structure Identification
Post-translational Modification Proteomics Analysis
Protein Interaction Mass Spectrometry Analysis
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