Cellular Proteomics

Cellular proteomics (Cellular proteomics) is a science that studies the expression, modification, interaction, and function of all proteins within a cell. As an important branch of proteomics, the development of cellular proteomics is closely related to the evolution of proteomics.In 1995, the term "proteomics" wasfirst introduced, marking the formal start of proteomics research. With advancements in mass spectrometry, research on intracellular proteins has become more refined. By theearly 21st century, cellular proteomics, as a subfield of proteomics, began to receive widespread attention. Since then, the introduction of new technologies and methods has continuously driven progress in this field, providing key information for the exploration of cell biology, disease mechanisms, and drug development.Currently, the main techniques in cellular proteomics include: two-dimensional gel electrophoresisand liquid chromatography for separating protein components into different subgroups to facilitate identification and quantification; mass spectrometry for identifying protein sequences and modification states; immunoprecipitationand affinity purification for capturing target proteins using specific antibodies or ligands to purify proteins; and bioinformatics analysis for integrating, mining, and visualizing omics data to reveal the biological significance of proteins.

BTPCellular Proteomics AnalysisGeneral Process1. ProteinExtraction:Breaking cells and removing non-protein components such as lipids, nucleic acids, and small molecule metabolites

;2. ProteinSeparation and Purification

:Using two-dimensional gel electrophoresis, liquid chromatography, and other methods to separate and purify protein samples;3. ProteinIdentification:

Using high-resolution tandem mass spectrometry(MALDI-TOF MS, ESI-MS/MS, etc.)to analyze the sequence and structure of the purified proteins;

4.ProteinQuantitative Analysis: Usingisotope labeling methods(such asiTRAQ, TMT, and SILAC, etc.),and label-free methods (such asLabel Free, SWATH-MS) to perform quantitative analysis, comparing protein expression differences under different conditions;5.

DataProcessing and Analysis: Using bioinformatics methods to process, analyze, and interpret mass spectrometry data, including protein identification, quantitative analysis, protein function annotation, interaction network analysis, pathway analysis, etc..OurAdvantages1.HighThroughputUsing efficient liquid chromatography and mass spectrometry technology, a large number of proteins can be identified and quantified in a short time.2.High EfficiencyCombining bioinformatics tools, large amounts of data can be mined, integrated, and visualized.3.Diversity

Applicable to multiple organisms and cell types, cellular proteomics analysis can be used tostudy protein changes under different environments, disease states, or genetic mutations.4.SystematicBy comprehensively revealing the expression, modification, and interaction of all proteins in a cell, it provides systematic and holistic information for biological research

.About Samples

Sample TypesOrganellesSecretions

Tissue SamplesBody Fluids

Tumor SamplesPathogen Samples

Embryo SamplesSample Quantity

100-1000mg1-100mg

1-100mg100-1000ug1-1000ug1-1000ug 

Chinese/English Project ReportIn the technical report, BTP provides a detailed bilingual technical report in Chinese and English, which includes:

1.Experimental Procedures (Chinese and English)2.

Related Mass Spectrometry Parameters (Chinese and English)

3.	Detailed Information on Cellular Proteomics Analysis	4.	Mass Spectrometry Images	5.	Raw Data		Cellular Proteomics
Analysis One-Stop Service	You only need to place an order and send the samples	BTP one-stop service completes: sample processing - on-machine analysis - data analysis - project report	Related Services	Cell Surface Proteomics	Subcellular ProteomicsSample ProteomicsExosome Proteomics	Proteomics4D Proteomics