Membrane Proteomics
Membrane proteins are a special class of proteins located on cell membranes that play a crucial role in many life processes, such as substance transport, signal transduction, energy conversion, and intercellular communication. Membrane proteomics is a science that studies the structure, function, interactions, and changes of membrane proteins in various biological processes. Because membrane proteins are embedded in a hydrophobic lipid bilayer, they are difficult to handle using traditional methods for separating and purifying water-soluble proteins. The extraction and purification of membrane proteins is therefore a daunting task. Additionally, the diversity and complexity of membrane protein structures make their identification and characterization more challenging. With the development of mass spectrometry, bioinformatics, and other technologies, these issues are gradually being resolved.
BTP Biotech adopts Thermo Fisher's Orbitrap Exploris 240 mass spectrometry platform combined with nanoLC-MS/MS nanoliter chromatography to provide one-stop subcellular proteomics analysis services, including qualitative and quantitative analysis of cell membrane proteomes, mitochondrial membrane proteomes, chloroplast membrane proteomes, etc.
General procedure of membrane proteomics analysis by BTP Biotech
1. Membrane protein isolation and enrichment
After cell or tissue separation and disruption, the cell membrane or subcellular structures are separated by centrifugation and membrane proteins are extracted for further separation and purification.
2. Protein digestion
Various proteases are used to digest membrane proteins into small peptide fragments for subsequent mass spectrometry analysis.
3. Peptide separation
The digested peptides are further separated and purified by high-performance liquid chromatography.
4. Mass spectrometry analysis
The separated and purified peptides are analyzed by mass spectrometry, and mass spectrometry data such as mass-to-charge ratios of peptide ions are recorded.
5. Mass spectrometry data analysis
The collected mass spectrometry data is combined with the corresponding protein database to achieve qualitative and quantitative identification of membrane proteins.
6. Bioinformatics analysis
Further analysis of the obtained protein data, such as protein classification, function prediction, pathway analysis, and subcellular localization.
Technical advantages of membrane proteomics analysis
1. Enables high-throughput and high-resolution identification of membrane proteins, including those with low abundance.
2. Multi-dimensional and multi-level techniques study membrane proteins, providing comprehensive information on membrane proteins.
3. Can be combined with other omics technologies such as transcriptomics and metabolomics to explore the functions and mechanisms of action of membrane proteins from a systems biology perspective.
About the sample
|
Sample types |
Cells |
Tissues |
Urine |
Serum, Plasma |
Microorganisms |
Protoplasts or Organelles |
|
Sample quantity |
10^6-10^7 cells |
50-100mg |
1-2ml |
50-200uL |
10^8-10^9 cells |
1-1000ug |
Bilingual project report in Chinese/English
In the technical report, BTP will provide you with a detailed bilingual technical report in Chinese and English, which includes:
1. Experimental procedures (in both Chinese and English)
2. Related mass spectrometry parameters (in both Chinese and English)
3. Detailed information on membrane proteomics analysis
4. Mass spectrometry images
5. Raw data
One-stop membrane proteomics analysis service
You only need to place an order and send samples
BTP's one-stop service completes: sample processing, on-machine analysis, data analysis, and project report
Related services
Quantitative proteomic analysis
Post-translational modification quantitative proteomics analysis
How to order?
