MS-MS Measurement of Protein Content and Purity

MS-MS involves selecting meaningful peptide ions for further secondary mass spectrometry analysis based on primary mass spectrometry. This means performing two consecutive mass spectrometry analyses on the sample to be tested, usually accomplished through tandem mass spectrometers such as Triple Quadrupole Mass Spectrometer (TQMS or QqQ), Hybrid Triple Mass Spectrometer Quadrupole Trap MS, and Hybrid Linear Ion Trap Orbitrap MS.

Mass spectrometry technology primarily identifies the relative molecular mass of proteins. By combining it with other techniques such as chromatography, it can characterize additional physicochemical properties, including protein amino acid sequence, content, concentration, purity, post-translational modifications, and protein-protein interactions. High-Performance Liquid Chromatography-Tandem Mass Spectrometry (HPLC-MS-MS) is an important mass spectrometry technology for analyzing protein content and purity. MS-MS couples two mass spectrometers, adding a collision reaction cell between the two quadrupoles to fragment precursor/parent ions selected by the first quadrupole, generating fragment/daughter ions. The secondary mass spectrometry further fragments ions broken down by the primary mass spectrometry, allowing for more detailed sample information with good reproducibility, resolution, and sensitivity, improving the accuracy of detection results.

Biotech company Biotai Parker utilizes Thermo Fisher's Q ExactiveHF mass spectrometry platform, combined with Nano-LC chromatography, to provide efficient and rapidprotein mass spectrometry analysisservice packages. You only need to inform us of your experimental objectives and send your cells to us. We will handle all subsequent project matters, including cell culture, protein extraction, protein digestion, peptide separation, mass spectrometry analysis, raw mass spectrometry data analysis, and bioinformatics analysis. Free consultation is welcome.

Related services:
Mass spectrometry for protein molecular weight determination
Protein molecular weight determination
Protein identification for gel spots, gel strips, and IP samples
Peptide mass spectrometry identification
Shotgun proteomics identification
Membrane protein identification service
Pull-down target protein mass spectrometry identification
Protein-protein interaction mass spectrometry analysis
Post-translational modification proteomics analysis
Protein sequencing
Protein N/C-terminal sequencing