Differential Proteomics

DifferenceProteomics

Differential ProteomicsIt is a branch of proteomics. Proteomics studies all proteins in a system, while differential proteomics aims to identify and characterize the differences and changes in protein groups between different samples or the same sample under different conditions.

During different growth, development, and physiological processes of organisms, the types and levels of proteins vary. Studying the composition and content of such protein groups, known as differential proteomics research, can reveal the essence of life activities, such as cellular regulation mechanisms and stress response pathways.

DifferenceProteomicsResearch Methods

Common methods for studying differential proteomics include two-dimensional electrophoresis, mass spectrometry, and Surface-Enhanced Laser Desorption/Ionization (SELDI) protein chip technology.

Two-dimensional electrophoresis is one of the most important techniques in differential proteomics research. It utilizes isoelectric focusing and polyacrylamide gel electrophoresis for two rounds of protein separation, followed by comparative analysis using a two-dimensional electrophoresis database.

Mass spectrometry can use stable isotope labeling to label two comparable samples, followed by mass spectrometry analysis of the mixed samples. This allows for qualitative and quantitative identification of proteins in the samples. Mass spectrometry is a powerful tool for studying differential proteomics due to its high resolution and sensitivity.

SELDI protein chip technology combines protein chips and mass spectrometry to analyze differential proteins in samples. Specially treated protein chips can selectively capture different types of proteins in samples, which are then eluted and analyzed by mass spectrometry. The biggest feature of SELDI protein chip technology is the ability to directly detect physiological fluids, such as blood, urine, tissue fluid, and cell lysates.

Based on research advancements in bioinformatics analysis methods, BGI Park Biotech has established a proteomics data bioinformatics analysis platform. This platform offers a comprehensive set of bioinformatics solutions, including quality analysis of omics data, differential expression analysis of omics data, GO, KEGG, and COG annotation analysis of differentially expressed proteins, protein clustering analysis, and multi-layer network and pathway analysis. We can also provide customized bioinformatics analysis services according to clients' needs and literature references, helping clients discover biological knowledge from high-throughput experimental data, aiding in drug design, toxicity analysis, and biomarker discovery. Feel free to contact us at 183-1097-1257 for a free consultation.

Submit Inquiry

Name *

Email Address *

Phone Number

Inquiry Project *

Project Description*

How to order?

Submit Inquiry