Immunopeptidomics Mass Spectrometry

Immunopeptides are non-classical peptides generated from sequences outside the protein-coding regions or through non-classical antigen processing mechanisms. They play a crucial role in the immune system's recognition of tumors and in cancer immunotherapy. Immunopeptidomics refers to the science of using mass spectrometry (MS) to study the composition and dynamics of immunopeptides presented by major histocompatibility complex (MHC) class I and II molecules. It enables large-scale, in-depth identification and quantification of HLA-presented immunopeptides and is a vital method for advancing cancer immunotherapy.

Mass spectrometry-based immunopeptidomics involves collective identification and quantification of peptide samples presented by human leukocyte antigen (HLA) molecules through immunoaffinity purification and high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) analysis. The identification of immunopeptides relies on mass spectrometry data combined with existing databases, which means it can only recognize peptide sequences already available in databases, posing challenges for new, unannotated atypical peptide antigens. It is worth mentioning that with the rapid development of DNA sequencing, RNA sequencing (RNA-seq), and ribosome sequencing (Ribo-seq) technologies, we can leverage genomic, transcriptomic, and translatomic data to expand the data interpretation for MS-based immunopeptidomics, providing guidance for immunopeptidomics analysis.

Biotech company Biotyper utilizes the Thermo Fisher Q ExactiveHF mass spectrometry platform combined with Nano-LC nano-chromatography, offering researchers proprietary, highly sensitive immunopeptide enrichment and identification solutions.Immunopeptidomics analysis based on high-precision mass spectrometryOne-stop technical service that can help you identify and recognize over 10,000 type I peptides and over 10,000 type II peptides, assisting researchers in finding solutions for cancer, immune diseases, and infectious diseases, and deeply exploring unknown targets. Free consultation available: 15201377680 (WeChat available).

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