Peptide Purity Determination

Peptides and proteins are compounds formed by the dehydration condensation of amino acids. They are mainly distinguished by molecular weight and the number of amino acids. Peptides can be obtained by the hydrolysis of proteins or synthesized in vitro. During in vitro synthesis or extraction of peptide samples from tissues, moisture or other inorganic salt impurities introduced during production, as well as chemical reagents from the extraction process, may be mixed in besides the target peptides. These impurities can affect subsequent peptide analysis, so it is necessary to detect the purity of peptides.

Peptide purity refers to the proportion of the target peptide in the sample relative to all substances in the sample at a wavelength of 220 nm (the maximum absorption peak of the peptide bond). Peptide purity is an important factor affecting experimental results; the lower the purity, the less stable and accurate the analysis results. Common methods for detecting peptide purity include reverse-phase high-performance liquid chromatography (RP-HPLC), high-performance liquid chromatography (HPLC), mass spectrometry, salting-out method, chromatography, and isoelectric precipitation method.

Biotechnology company Biotyper uses Thermo Fisher's Q ExactiveHF mass spectrometry platform combined with Nano-LC nano-chromatography to provide highly efficient and precisepeptide purity determinationservice packages, which can perform purity testing on various peptide samples and offer customized detection solutions based on requirements. Free consultations are welcome.

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