Peptide Structure Identification

Polypeptides are compounds composed of amino acids, similar to proteins, and have various spatial structures like proteins, such as primary, secondary, and more advanced spatial structures. The structure of polypeptides, especially the primary structure, i.e., the composition and sequence of amino acids, is important reference information for the qualitative identification of polypeptides. The advanced structure to some extent determines its biological function. Therefore, structural identification of polypeptides helps in qualitative analysis and elucidation of their biological functions.

The structural identification of polypeptides, especially long-chain polypeptides, is a rather important and complex task. Currently, there are many methods available for polypeptide structural identification, such as the traditional Edman degradation method, C-terminal enzymatic method, and emerging techniques like mass spectrometry, nuclear magnetic resonance, infrared/ultraviolet spectroscopy, circular dichroism spectroscopy, and X-ray crystallography. These methods have different principles, advantages, and disadvantages, and primarily identify different types of structures. For example, Edman degradation, C-terminal enzymatic, and mass spectrometry mainly determine primary structures, while nuclear magnetic resonance, infrared/ultraviolet spectroscopy, circular dichroism spectroscopy, and X-ray crystallography can identify more advanced polypeptide structures. In practical applications, it is essential to choose an appropriate detection method based on the specific experimental objectives.

Biotech company Biotech Park uses Thermo Fisher's Q Exactive HF mass spectrometry platform, combined with Nano-LC nano-liquid chromatography, to provide efficient and accurateprotein/polypeptide structure identificationservice technical package, which can analyze the primary, secondary, and other structures of various protein/polypeptide samples, including the types, numbers, and sequence of amino acids in the polypeptide chain, as well as the positions and numbers of disulfide bonds within or between polypeptide chains. We welcome free inquiries.

Related services:
Protein structure identification
Protein circular dichroism analysis
Determination of protein primary structure
Detection of disulfide bonds/free cysteine in biopharmaceuticals
Identification and quantitative analysis of protein disulfide bonds
Amino acid composition analysis
Sequence analysis based on mass spectrometry