Difference between Pull Down and Immunoprecipitation

Co-Immunoprecipitation (Co-IP) can detect the specific interaction between two proteins in vitro. Co-IP lyses cells under non-denaturing conditions, preserving many intracellular protein interactions. If protein X can specifically bind to an antibody, then protein Y, which interacts with protein X, will precipitate together with protein X through the specific recognition of the antibody. By studying protein Y, the interaction between proteins X and Y can be confirmed. In Co-IP analysis, the bait and prey proteins are in their native conformational state, interactions occur in vivo, are less affected by external influences, and do not require cloning and heterologous expression, only the selection of suitable antibodies is needed to start the experiment.

Pull Down is an in vitro affinity purification method that uses the bait protein to enrich proteins that interact with it. Immunoprecipitation uses antibody proteins to recognize and bind interacting proteins X and Y, while Pull Down directly recognizes and binds target proteins that can interact with the bait protein. It has the ability to purify low-abundance protein complexes and is commonly used in in vivo and in vitro transcription systems.

Biotech company Baitai Pak uses Thermo's newly launched Obitrap Fusion Lumos mass spectrometer combined with Nano-LC to provide Pull Down and Co-IP basedprotein interaction analysisservice package, which can identify the potential direct interaction between two known proteins of interest and search for unknown proteins that may interact with the target protein. Free consultation is welcome.

Submit Inquiry

Name *

Email Address *

Phone Number

Inquiry Project *

Project Description*

How to order?

Submit Inquiry