Introduction to the Types and Principles of Glycosylation
Glycosylation is one of the types of post-translational modifications that occur in the endoplasmic reticulum and Golgi apparatus of the cell. Glycosylation modification is the process of attaching sugars to proteins or lipids under the action of glycosyltransferases. It is an important protein modification that regulates protein function. Below, the types and principles of glycosylation will be introduced.
The principle of glycosylation is that glycans are transferred to proteins under the action of glycosyltransferases, connecting with amino acid residues on the proteins to form glycosidic bonds. Proteins modified by glycosylation are called glycoproteins. This process is regulated by the control of enzymes. Glycosylation is a post-translational modification that involves the addition of sugars to immature proteins through the action of related enzymes, promoting the proper folding of proteins, thereby increasing protein stability and producing functional proteins.
Glycosylation types can be divided into four categories based on the way the glycan chains are linked to proteins in glycoproteins, namely N-glycosylation, O-glycosylation, C-glycosylation, and GPI glycosylphosphatidylinositol. Among these, N-glycosylation and O-glycosylation are the main types of modifications. N-glycosylation is the most widespread in organisms, followed by O-glycosylation.
Biotech company Biotech Peak uses MALDI TOF MS high-resolution mass spectrometry and UHPLC technology to provide Glycomics analysis services. This service can be used to identify glycosylation modifications expressed in plasma/serum, cells, tissues, or organisms. All glycans connected to proteins will be hydrolyzed by enzyme digestion, separated by hydrophilic chromatography, and quantified using a mass spectrometry system. You only need to inform us of your experimental objectives and send the samples, and we will take care of all subsequent project matters, including protein extraction, proteolysis, peptide separation, mass spectrometry analysis, raw mass spectrometry data analysis, and bioinformatics analysis.
Related services:
Quantitative glycoproteomics analysis
Glycosylation site and glycan type analysis
N-glycan modification and modification site analysis services
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