- Primary Structure Analysis
- High-resolution mass spectrometry molecular weight
- MALDI TOF mass spectrometry analysis
- N-terminal sequence analysis
- C-terminal sequence analysis
- N/C terminal sequence analysis
- Analysis of the K deletion ratio at the C-terminus of antibodies
- LC-MS/MS protein full sequence validation
- Peptide coverage / Peptide spectrum analysis
- Protein peptide profile determination
- Amino Acid Composition Analysis
- Extinction coefficient analysis
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- Advanced Structural Analysis
- Charge Heterogeneity Analysis
- Impurity Analysis
- Native Mass Spectrometry
- SDS-PAGE protein purity analysis
- Protein purity analysis (size exclusion/reverse phase chromatography)
- Host Cell Protein Residue (HCP) Analysis Service
- Antibody-Drug Conjugates (ADCs) Analysis
- Protein content analysis
- Product-related impurity analysis
- Analysis of other process-related impurities
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- Special Analysis
- Primary Structure Analysis
The Sephadex G-100 protein purification column is very clogged, and the flow rate is extremely slow. How can I solve this?
When using Sephadex G-100 to purify proteins, if you encounter issues with column blockage and extremely slow flow rates, you can try the following methods to resolve the problem:
1. Check the column packing:
Ensure that the Sephadex G-100 medium is packed uniformly without air bubbles. Air bubbles or uneven packing can affect column flow.
2. Adjust the sample volume and concentration:
If the sample volume is too large or the concentration too high, it can cause column blockage. Try reducing the sample load or diluting the sample.
3. Increase the buffer strength:
Sometimes increasing the buffer strength can help improve flow, for example, by increasing the salt concentration in the buffer.
4. Gently wash the column:
If the column is already blocked, try gently washing it with a buffer of higher salt concentration or a buffer containing detergents.
5. Re-pack the column:
If the above methods do not solve the problem, it may be necessary to re-pack the column. This includes washing and re-equilibrating the Sephadex G-100 medium.
6. Check other parts of the system:
Ensure all connections and tubing are free from blockages or leaks, as these can also slow down the flow rate.
If the problem persists, consider consulting the manufacturer or seeking professional technical support.
Biotech Pack BioTech -- A leading service provider for bioproduct characterization and multi-group biomolecular mass spectrometry analysis
Related services:
Protein purity and homogeneity characterization
SDS-PAGE protein purity analysis
Protein purity analysis (size exclusion/reverse phase chromatography)
Protein isoelectric point determination
Protein structure identification
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