ProExcell^TM Bacterial Protein Extraction Kit

	Product Name: ProExcell TM Bacterial Protein Extraction Kit
	Catalog Number: BTP-P97002
	Specifications: 25-100T
	Product Type: Protein Extraction Kit
	Order/Inquiry Hotline: 011-57231915 152-0137-7680
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Catalog Number	BTP-P97002	Bacteria, especially E. coli, are commonly used as protein expression vectors in genetic engineering. Bacterial protein extraction is a critical process for isolating and purifying recombinant proteins expressed by specific bacteria, and it is the final step to obtain the desired target protein. This process holds significant value for scientific research, pharmaceutical development, and industrial applications. However, current ultrasonic extraction and glass bead grinding methods still have many shortcomings in terms of protein yield and the content of impurities like host cell DNA and endotoxins in the lysate.
Specifications	25-100T
Product Type	Protein Extraction Kit
Storage Conditions	Refrigerated

Challenges in Bacterial Protein Extraction

1) Cell Wall Structure: The peptidoglycan in bacterial cell walls may form a barrier in the protein extraction process, hindering protein release and extraction.

2) Solubility Issues: Many recombinant proteins expressed in bacteria may form insoluble, highly aggregated inclusion bodies, making their separation and resolubilization challenging.

3) Endogenous Protein Contamination: Proteins expressed by bacteria themselves may mix with the target protein, complicating the purification process.

4) Protein Abundance: For some recombinant proteins, especially those toxic to bacteria themselves, expression levels may be low, requiring more sensitive and efficient extraction methods.

5) Protein Stability: Recombinant proteins may be unstable and degrade during extraction and purification, leading to low extraction efficiency.

6) Purification Complexity: Some target proteins may require complex purification schemes, such as affinity chromatography and ion exchange chromatography, increasing the complexity and cost of the process.

7) Rich in Impurities: Impurities like endotoxins and DNA from bacterial cells may affect the purity and accuracy of recombinant protein extraction.

After multiple rounds of experimental optimization and validation, Bio-Tech Pack (BTP) has developed the highly efficient ProExcell ^TM bacterial protein extraction kit, providing you with a complete set of bacterial protein extraction reagents and methods to effectively extract proteins from bacteria. This kit achieves high extraction efficiency and reduces protein degradation during extraction, offering feasible solutions for obtaining high-purity, efficient, rapid, and stable and reliable samples in sample pretreatment and protein extraction.

ProExcell ^TM Advantages/Features of the Bacterial Protein Extraction Kit

Bio-Tech Pack ProExcell ^TM bacterial protein extraction kit has undergone multiple rounds of optimization by our company and has the following outstanding product advantages:

1. Mass Spectrometry Grade Protein Extraction: Our kit has been rigorously optimized and validated to extract proteins with purity reaching mass spectrometry grade . Whether for proteomics research, protein modification analysis, or other mass spectrometry applications, our kit meets the requirements for high purity and high sensitivity.

2. Extraction Efficiency: ProExcell ^TM bacterial protein extraction kit employs an optimized extraction method, enhancing protein extraction efficiency to ensure sufficient protein is obtained from limited samples. This is especially important in cases of limited or rare samples. Compared to other leading commercial lysis reagents, the soluble protein extraction efficiency is increased by 10 times .

3. Highly Efficient and Fast: Our kit's operation process is highly efficient, requiring no ultrasonic treatment or freeze-thaw cycles , not only saving experimental time but also improving the efficiency of sample processing, saving your research valuable time and resources.

4. Optimized Extraction Scheme: Our product has undergone multiple rounds of experimental optimization to ensure the best protein extraction scheme , allowing proteins to be fully dissolved and released from samples while ensuring that the functional integrity of the dissolved proteins is maintained .

5. Simple and Convenient: The cell lysates obtained from our kit are easy to use, requiring no addition of nucleases.

6. Low Interference: Our product has been optimized to minimize the viscosity generated by DNA and RNA release .

7. High Throughput: Our kit is suitable for large-scale protein extraction or high-throughput cell lysis .

8. High Consistency and Repeatability: Our product undergoes strict quality control to ensure the stability and consistency of reagents. You can obtain consistent results under different experimental conditions, ensuring the reliability and repeatability of data .

9. Compatibility: ProExcell ^TM bacterial protein extraction kit's extracted proteins have strong compatibility, compatible with protease inhibitors, salts, chelating agents, and reducing agents , allowing direct use in subsequent analyses such as mass spectrometry, western blotting, ELISA, and more without additional treatment.

10. Customization Support: We understand that each laboratory and research project has unique needs, so we offer personalized technical support and solutions. Our team is willing to work closely with you to ensure you can fully utilize our products and achieve the best research results.

ProExcell ^TM bacterial protein extraction kit provides mass spectrometry-grade protein extraction capability, efficient and gentle, enabling the extraction of soluble proteins from bacterial cells most effectively. It can perforate bacterial cell walls without denaturing proteins. ProExcell ^TM bacterial protein extraction kit's unique and gentle formulation minimizes interference with binding and elution processes, ensuring excellent purification results.

ProExcell ^TM Product Applicability of the Bacterial Protein Extraction Kit

Our ProExcell ^TM bacterial protein extraction kit enables high-purity extraction of proteins from various bacteria, especially E. coli. Feel free to consult us for free!

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