ProExcell^TM Histone Extraction Kit

	Product Name: ProExcell TM Histone Protein Extraction Kit
	Catalog Number: BTP-P97009
	Specifications: 25-100T
	Product Type: Protein Extraction Kit
	Order/Consultation Hotline: 011-57231915 152-0137-7680
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Catalog Number	BTP-P97009	Histone proteins, as the core proteins wrapped around chromosomal DNA, are key regulatory factors of cellular genetic information. Their modifications, such as acetylation and methylation, are closely related to the expression of many genes, DNA repair, and the maintenance of chromosome structure. Identifying these covalent modifications on typical and variant histones is crucial for understanding their biological significance. Therefore, an extraction method that can effectively isolate histones while preserving their modifications is essential for epigenetic studies of these proteins.
Specifications	25-100T
Product Type	Protein Extraction Kit
Storage Conditions	Refrigerated

Challenges in Histone Protein Extraction

1) Close Association with DNA: Histone proteins are nuclear proteins closely associated with DNA. Extracting these proteins requires using high salt or specific chemical reagents to neutralize their electrostatic interactions with DNA, promoting the dissociation of histones.

2) Complexity of Sample Processing: To extract histones, the nucleus must first be separated from the cytoplasm, and then the nucleus must be broken down to release chromatin. This series of steps requires careful handling to ensure the integrity and functionality of the proteins.

3) Complexity of Covalent Modifications: Most histone proteins undergo one or more covalent modifications, such as acetylation, methylation, and phosphorylation. These modifications are crucial for studying histone function, so it is important to ensure that these modifications remain intact during extraction.

4) Abundant Impurities: Besides histones, there are many other nuclear proteins and non-histone proteins in the nucleus. These may mix with the extracted histones, leading to insufficient purity and interfering with subsequent experiments.

5) Extraction Efficiency: Due to the close association of histones with DNA, inappropriate extraction conditions may result in incomplete extraction of some histones, reducing extraction efficiency.

To address these technical challenges and better analyze histone functions and their modifications, BiotaiPack Biotechnology (BTP) has developed the ProExcell ^TM histone protein extraction kit, designed to provide an efficient, stable, complete, and reliable histone protein extraction solution for the core part of modern biological research—histone studies.

ProExcell ^TM Advantages/Features of the Histone Extraction Kit

BiotaiPack Biotechnology ProExcell ^TM histone extraction kit has undergone multiple rounds of research and optimization, featuring the following outstanding product advantages:

1. Efficient Dissociation from DNA: Our kit includes specific chemical reagents, ensuring effective nuclear lysis and dissociation of interactions between histones and DNA , maximizing histone protein extraction.

2. Reduced Impurity Interference: Our product's extraction process is optimally designed to effectively remove and reduce non-histone proteins and other impurities , resulting in high-purity histone samples.

3. Stability of Modifications Maintained: Our kit uses specific proteases and inhibitors to preserve histone modification states , ensuring that protein modifications are not disrupted or lost during extraction. With an optimized protocol, it effectively recovers core histones (including histones H3, H4, H2A, and H2B). .

4. High Efficiency and Speed: Our kit is designed for simplicity, with an efficient process that allows rapid acquisition of high-purity histones from samples. This not only saves experimental time but also improves processing efficiency, conserving valuable time and resources for your research.

5. Mass Spectrometry Grade Protein Extraction: Our kit has been rigorously optimized and validated to extract proteins with purity reaching mass spectrometry grade . Whether for proteomics research, protein modification analysis, or other mass spectrometry applications, our kit meets the requirements for high purity and high sensitivity.

6. High Consistency and Reproducibility: Our product undergoes strict quality control to ensure reagent stability and consistency. You can achieve consistent results under different experimental conditions, ensuring data reliability and reproducibility .

7. Compatibility: ProExcell ^TM histone protein extraction kit provides proteins with high compatibility, compatible with protease inhibitors, salts, chelating agents, and reducing agents , without requiring additional processing of the extracted proteins, allowing direct use in various subsequent experimental analyses, including mass spectrometry, immunoblotting, ELISA, etc.

8. Customized Support: We understand that every laboratory and research project has unique needs, so we offer personalized technical support and solutions . Our team is willing to work closely with you to ensure you can fully leverage our products and achieve the best research results.

ProExcell ^TM histone protein extraction kit overcomes multiple technical challenges and has mass spectrometry-grade protein extraction capabilities, suitable for a series of subsequent mass spectrometry-related analyses. Whether you are assessing post-translational modifications of histones in normal and diseased tissues or screening drug compounds in cultured cells, BiotaiPack Biotechnology ProExcell ^TM histone protein extraction kit is your best choice for preparing histone protein samples.

ProExcell ^TM Histone Protein Extraction Kit Product Applicability

Our ProExcell ^TM histone protein extraction kit enables efficient extraction of histones from various samples such as cells, fungal mycelia, plant leaves, and kelp. Feel free to inquire for free!

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