ProExcell<sup>TM</sup> Fungal Protein Extraction Kit
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Product Name: ProExcell TM Fungal Protein Extraction Kit |
Item Number: BTP-P97007 | |
Specification: 25-100T | |
Product Type: Protein Extraction Kit | |
Order/Inquiry Hotline: 011-57231915 152-0137-7680 |
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Item Number | BTP-P97007 |
Fungi contain many different proteins, and they are abundant, comprising 31% of the dry weight of mushrooms. The proteins in fungi contain all essential amino acids, and their composition is similar to animal proteins, making them a high-quality protein source. In recent years, due to their high nutritional value, production efficiency, and low environmental impact, fungal proteins have become a research and market hotspot as an alternative to animal-derived proteins and other plant proteins. To release fungal proteins, powerful cell disruption and extraction technologies are needed to break the indigestible cell walls. Researching various cell wall degradation and fungal protein extraction methods could increase the potential applications of fungal proteins in food. |
Specification | 25-100T | |
Product Type | Protein Extraction Kit | |
Storage Conditions | Refrigerated |
Specific Challenges of Fungal Protein Extraction
1) Hard Cell Walls: Unlike other biological cells, fungal cell walls are more complex, usually composed of cellulose, chitin, and glucan, making them harder to break to release proteins.
2) Rich Endomembrane System: Fungal cells contain many endomembranes, and the proteases in these endomembranes may degrade target proteins during extraction.
3) Activity of Intracellular Enzymes: During fungal cell disruption, intracellular enzymes such as proteases may be released, potentially degrading the extracted proteins.
4) Choice of Extraction Buffer: To ensure protein quality and quantity, different fungal species and research purposes may require different extraction buffers.
5) Solubility of Proteins: Some fungal proteins may be difficult to dissolve during extraction or may precipitate in subsequent steps, requiring optimization of extraction and purification conditions.
6) Removal of Contaminants: Unwanted cell components such as nucleic acids, polysaccharides, and small molecule metabolites may be introduced during extraction, potentially affecting subsequent experimental analyses.
ProExcell TM Advantages/Features of the Fungal Protein Extraction Kit
BTP Biotech's ProExcell TM Fungal Protein Extraction Kit has undergone multiple rounds of optimization and has the following outstanding product advantages:
1. Targeted Cell Wall Disruption: Our kit contains components specifically targeting fungal cell wall structures, ensuring complete cell wall breakage to release internal proteins.
2. Efficient Separation Technology: ProExcell TM The kit's optimized separation technology ensures that proteins from subcellular organelles such as vacuoles and vesicles can also be effectively extracted.
3. Extraction Efficiency: ProExcell TM The fungal protein extraction kit uses optimized extraction methods with fungal-specific protease inhibitors to increase protein extraction efficiency, ensuring sufficient protein is obtained from limited samples, particularly important when sample amounts are limited or rare. Compared to other leading commercial lysis agents, soluble protein extraction efficiency is increased by 10 times .
4. Highly Efficient and Fast: Our kit's operation process is efficient, requiring no ultrasonic treatment or freeze-thaw processing , not only saving experimental time but also improving sample processing efficiency, saving valuable time and resources for your research.
5. Optimized Extraction Plan: Our product has undergone several rounds of experimental optimization to ensure the best protein extraction plan , allowing proteins to dissolve and release fully in the sample while ensuring the functional integrity of the dissolved proteins .
6. Preservation of Protein Modification States: Specific stabilizers in our kit help maintain the original state of proteins , including their unique post-translational modification states, ensuring protein integrity.
7. Mass Spectrometry-Grade Protein Extraction: Our kit has been rigorously optimized and validated to extract proteins with purity reaching mass spectrometry-grade levels . Whether for proteomics research, protein modification analysis, or other mass spectrometry applications, our kit meets high purity and sensitivity requirements.
8. High Consistency and Reproducibility: Our product undergoes strict quality control to ensure reagent stability and consistency. You can achieve consistent results under different experimental conditions, ensuring data reliability and reproducibility .
9. Compatibility: ProExcell TM The proteins extracted with the fungal protein extraction kit have strong compatibility, compatible with protease inhibitors, salts, chelating agents, and reducing agents , requiring no additional treatment of the extracted proteins for multiple subsequent experimental analyses, including mass spectrometry, immunoblotting, ELISA, etc.
10. Customized Support: We understand each laboratory and research project has unique needs, so we offer personalized technical support and solutions. Our team is willing to work closely with you to ensure you can fully utilize our products and achieve the best research results.
ProExcell TM The fungal protein extraction kit fully considers the uniqueness of fungal cells and the needs of researchers, providing a highly efficient and reliable solution for laboratories. Its strong performance ensures the integrity and functionality of fungal proteins, laying a solid foundation for subsequent research and applications.
ProExcell TM Product Applicability of Fungal Protein Extraction Kit
Our ProExcell TM Fungal Protein Extraction Kit has broad applicability and compatibility, especially for yeast and Pleurotus ostreatus, achieving efficient and rapid protein extraction. Feel free to inquire for more information!
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