CE-SDS Protein Isomerization Analysis

Protein isomerization analysis is a method used to study the differences in structure and function of proteins. Proteins are key molecules in life activities, and their functions are closely related to their structures. However, the same amino acid sequence may exist in multiple three-dimensional forms known as isomers. Protein isomerization can be caused by various factors such as post-translational modifications, heterologous expression, environmental factors, etc. The isomerization of monoclonal antibodies is one of the common pathways of protein degradation. Isomers may not only lead to changes in protein function but are also associated with various diseases, such as neurodegenerative diseases and cancer. Therefore, analyzing and identifying protein isomerization is crucial, especially in drug development and protein drug production.

 

Different protein isomers encoded by the same gene

 

Capillary Electrophoresis-Sodium Dodecyl Sulfate (CE-SDS) technology is an effective method for detecting and distinguishing protein isomers. Due to differences in charge, size, and shape among protein isomers, they have different migration rates in an electric field, allowing for their separation, detection, and identification. The advantages of this technology lie in its ability to analyze purified protein samples as well as complex biological samples such as cell lysates or tissue extracts. The high resolution of CE-SDS enables it to distinguish very similar isomers, and its high sensitivity, high resolution, and rapid nature provide a powerful tool for the structural and functional study of protein isomers.

 

Based on high-precision capillary electrophoresis instruments and protein electrophoresis principles, BTP (Biotechnology) has developed and validated a CE-SDS protein isomerization analysis platform. This platform provides CE-SDS protein isomerization analysis services that comply with CNAS/ISO9001 dual quality system standards, and it can be used to analyze the isomerism of protein products such as monoclonal monomers under normal and stress conditions, aiding in the early identification of easily degradable candidate drugs during drug development.

 

Project report in Chinese/English

 

In the technical report, BTP will provide you with a detailed bilingual version (Chinese and English) of the technical report, which includes:

 

1. Experimental procedures (in Chinese and English)

2. Relevant experimental parameters (in Chinese and English)

3. Detailed information on protein isomers

4. Electrophoresis images

5. Raw data

 

CE-SDS Protein Isomerization AnalysisOne-stop service

 

You only need to place an order and send the samples

 

BTP's one-stop service completes: sample processing, on-machine analysis, data analysis, and project report

 

Related services

Protein structure identification
Protein circular dichroism analysis
Determination of protein primary structure