Peptide Structure Determination

Polypeptides are widely present in organisms and participate in manyimportantlife activities, such as hormones, enzymes, antigens, antibodies, etc. The spatial structure and functional characteristics of polypeptides have a crucial impact on their biological activity. Accurately determining the structure of polypeptides is essential forrevealing the structural characteristics, folding patterns, and biological activities of polypeptides, understanding their mechanisms of action, developing drug treatment strategies, and optimizing bioengineering.The determination of polypeptide structure holds significant value in fields such as biological science, medical research, and drug development. It helps in deepening the understanding of the relationship between protein structure and function, provides a basis for new drug design, and promotes vaccine research and development. Additionally, polypeptide structure analysis is crucial for studying disease mechanisms, developing protein drugs, and optimizing biotechnological applications.

 

Biotai PikeBiotechnology BTPPolypeptide structure determinationprocess

1. Sample preparation:

Target polypeptides are extracted and purified using methods such as high-performance liquid chromatography (HPLC). The purified polypeptides are desalted and concentrated to meet the requirements of structural determination.

 

2. Sequence analysis:

a.Choose appropriate enzymes based on the characteristics of the polypeptide sequence to cleave the sample into peptides suitable for mass spectrometry analysis.

b. Usehigh-performance liquid chromatography (HPLC) to separate the peptides produced by enzymatic digestion.

c.Determine the amino acid composition and sequence of peptidesusingEdman degradationor tandem mass spectrometry(MS/MS)techniques,obtainingthe primary structure of the polypeptide. 

3. Structure analysis:

Measure the absorption of polypeptides in the ultraviolet region

usingcircular dichroism spectroscopyto determineinternal secondary structural elements such as α-helices and β-sheets. 

Main applications of polypeptide structure determination

1. Reveal protein structure:

Polypeptide structure determination can help identify the three-dimensional structure of proteins, including their main conformation, secondary, tertiary, and quaternary structures.

 

2. Study protein function:

The structure and function of proteins are closely related, and polypeptide structure determination can reveal the connection between protein structure and function.

 

3. Develop new drugs:

Polypeptide structure determination can help identify the mode of interaction between drugs and target proteins, providing important information for new drug design.

 

4. Disease diagnosis and treatment:

Polypeptide structure determination helps discover biomarkers associated with diseases, improving early diagnosis and treatment efficacy.

 

Bilingual project report

In the technical report, Biotai Pike provides you with a detailed bilingual technical report in both Chinese and English, including:

1. Experimental steps (Chinese and English)

2. Relevant mass spectrometry parameters (Chinese and English)
3.
Detailed information on polypeptide structure determination 4. Mass spectrometry images
5. Raw data
 

One-stop service for polypeptide structure determination

You only need to place an order and send the samples

Biotai Pike provides a one-stop service: Sample processing - Instrumental analysis - Data analysis - Project report
 

Related services

Protein circular dichroism analysis

Determination of primary protein structure
Detection of biopharmaceutical disulfide bonds/free cysteine
Identification and quantification of protein disulfide bonds
Polypeptide mass spectrometry identification
Polypeptide omics analysis
Polypeptide sequencing
De novo peptide sequencing