Deamidation Modification Analysis
Protein deamidation refers to the process where amidated amino acids in protein molecules, such as glutamine and asparagine, lose an ammonia molecule (NH3) under specific conditions, converting them into their corresponding acids, i.e., α-ketoglutaric acid and glutamic acid. Deamidation is a non-enzymatic post-translational modification, which is crucial for understanding protein charge distribution, function, stability, interactions with other molecules, and its relation to diseases. For example, β-amyloid protein associated with Alzheimer's disease may form fibrils more readily after deamidation. With the advancement of molecular biology and mass spectrometry technologies, analyzing protein deamidation modifications has become possible. Mass spectrometry can accurately identify specific sites in protein sequences that are subject to deamidation modifications, providing key information for studying protein function, structure, and interactions.233.
Asai, H. K. et al.AppliedChem. 2021.
Protein Deamidation Modification Process
Protein deamidation mass spectrometry analysis is an advanced method for studying proteins. This technique uses specific enzymes or chemical reagents to induce deamidation reactions in proteins, converting the carboxyl groups of asparagine and glutamine into amide groups. After deamidation, the mass of these amino acids is reduced by approximately 1 Da, creating recognizable mass-to-charge ratio differences in subsequent mass spectrometry analysis. Through these differences, we can accurately identify and quantify deamidation modifications in proteins. Based on high-resolution mass spectrometry, Biotech Pack (BTP) has established an efficient platform for protein deamidation modification analysis, certified by CNAS/ISO9001 dual quality systems, providing you with quality-assured one-stop protein deamidation modification mass spectrometry analysis services, supporting research in cellular signal transduction, protein interactions, and disease mechanisms.
Chinese/English Project Report
In the technical report, Biotech Pack provides you with a detailed bilingual technical report in Chinese and English, including:
1. Experimental procedures (Chinese and English)
2. Relevant experimental parameters (Chinese and English)
3. Detailed information on protein deamidation modification
4. Mass spectrometry images
5. Raw data
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