Amino Acid Sequence Determination

Amino Acid Sequence Determination

The amino acid sequence refers to the order of amino acids in a protein or polypeptide molecule. It is a part of the primary structure of proteins and largely determines the higher-order structures and biological functions of proteins or polypeptides. Therefore, amino acid sequence determination helps in studying the mechanisms of functional proteins or active polypeptides and provides a basis for the artificial synthesis of active polypeptides.

Methods of Amino Acid Sequence Determination

The main methods for amino acid sequence determination include traditional Edman degradation sequencing and mass spectrometry:

The traditional Edman degradation sequencing method is cumbersome and cannot determine sequences with blocked or modified N-termini.

Mass spectrometry-based sequencing methods determine the molecular mass of amino acids by analyzing the mass-to-charge ratio spectra of ions. Combined with corresponding databases, the type of amino acids can be inferred. Mass spectrometry is simple, fast, highly sensitive, and can analyze sequences with blocked or modified N-termini, thus overcoming some limitations of Edman sequencing.

Biotech company Biotye based on Thermo's newly launched Obitrap Fusion Lumos mass spectrometer provides one-stop amino acid sequence analysis services, capable of conducting amino acid composition and sequence identification for various protein/polypeptide samples. Free consultation is welcome at 152-0137-7680.

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