Acetylated Protein WB

Acetylation modification is a reversible and highly conserved post-translational modification of proteins, often occurring in histones and non-histones. It plays an important regulatory role in gene expression, transcription factor activity, cellular metabolic pathways, and metabolic enzyme activity. WB (Western blotting), also known as immunoblotting or protein blotting, is an immunochemical technique developed on the principle of specific antigen-antibody binding, combining gel electrophoresis and immunochemical techniques to detect a specific protein in complex samples, allowing for qualitative and semi-quantitative analysis.

Acetylated protein WB uses immunoblotting technology to detect acetylated proteins. The general experimental procedure involves transferring protein samples separated by PAGE to a solid-phase carrier. The proteins on the solid-phase carrier act as antigens that immunologically bind to the corresponding antibody (primary antibody), then specifically bind to a specially labeled secondary antibody. Finally, detection is performed using substrate coloration or autoradiography techniques, enabling qualitative identification of acetylated proteins.

Biotechnology company Baite Parker uses Thermo Fisher's Q Exactive HF mass spectrometry platform combined with Nano-LC to provideProtein Acetylation Modification Analysisservice technology package. You only need to tell us your experimental purpose and send your samples to us. We will handle all subsequent project matters, including protein extraction, protease digestion, acetylated peptide enrichment, peptide separation, mass spectrometry analysis, raw mass spectrometry data analysis, and bioinformatics analysis. Free consultation is welcome.

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