TMT Labeling Quantitative Proteomics

TMTLabelQuantitativeProteomics Definition

Quantitative proteomics techniques are mainly divided into labeled and label-free categories. Labeled quantitative proteomics techniques can be further divided into in vivo labeling and in vitro labeling. TMT (Tandem Mass Tag) is an in vitro peptide labeling technique. TMT-labeled quantitative proteomics utilizes TMT technology to accurately identify and quantify all proteins expressed by a genome of an organism or all proteins in a mixed system, revealing protein changes during a specific life activity of the organism.

TMTLabelQuantitativeProteomics Research Methods

TMT reagent labels consist of three parts: an amine-reactive group, a mass reporter group, and a mass normalization group. The amine-reactive group specifically recognizes the N-terminus of peptides and the side chain of lysine, forming a covalent bond, thereby achieving isotopic labeling. The mass reporter group and mass normalization group each have 10 different molecular weights, which result in the same mass-to-charge ratio for the same peptide segment from different sources labeled with TMT in the first mass spectrometry. In the second mass spectrometry, TMT reporter ions are released through fragmentation, allowing the ion peak intensity to be analyzed against the mass-to-charge ratio. Using software combined with the appropriate database, the ion peak intensity and mass-to-charge ratio values can be analyzed to calculate the protein content of the sample.

Biotech company Baitai Parker uses Thermo's latest Obitrap Fusion Lumos mass spectrometer combined with Nano-LC to provideTMT Quantitative Proteomics Analysisone-stop service. Feel free to inquire at 183-1097-1257.

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