De novo protein sequencing data analysis

Protein sequencing involves determining the amino acid sequence of a protein using methods such as mass spectrometry. De novo protein sequencing involves identifying amino acid information by analyzing the regular fragment ions produced when protein peptides collide with inert gases during mass spectrometry detection, and then inferring the amino acid sequence through the mass differences between adjacent ions.

De novo protein sequencing data analysis does not rely on or reference any known amino acid sequences or database information. It directly analyzes the amino acid data of peptides detected by mass spectrometry and then uses computer algorithms and bioinformatics analysis for amino acid sequence assembly and annotation to ultimately obtain the complete protein amino acid sequence. The obtained protein amino acid sequence can be quality controlled using indicators such as FAR (False Amino-acid Rate) and FDR (False Discovery Rate) to ensure a high-confidence, correct target protein amino acid sequence.

Biotechnology company Biotage employs the Thermo Obitrap Fusion Lumos mass spectrometer combined with Nano-LC nanoliter chromatography technology to providemass spectrometry-based peptide de novo sequencingservices for peptide sequence analysis. The Obitrap Fusion Lumos mass spectrometer is currently the highest resolution and sensitivity mass spectrometer, ensuring the sensitivity of low-abundance peptide fragment identification. It employs a combination of HCD and ETD modes during peptide fragmentation to ensure the integrity of peptide fragments. After obtaining the raw mass spectrometry data, de novo sequencing is used to deduce the peptide sequence.

Related services:

• De novo sequencing
• Singlemonoclonal antibody de novo sequencing
• Protein de novo sequencing and mutation analysis
• Sequence analysis
• Mass spectrometry-based sequence analysis
• Protein N-terminal sequencing based on Edman degradation
• Monoclonal antibody sequencing service based on PCR amplification
• Protein sequencing based on the Top-down approach
• Protein sequencing
• Peptide sequencing
• Antibody sequencing