Can ultraviolet-visible spectrophotometry be used for quantitative analysis of amino acids and proteins?
Yes, UV-Vis spectrophotometry is a common technique used for the quantitative analysis of amino acids and proteins.
1. Quantitative analysis of amino acids:
Certain amino acids, such as tryptophan and tyrosine, can absorb UV light, particularly around 280 nm. Therefore, UV-Vis spectrophotometry can be directly used for their quantitative analysis. However, for amino acids that do not absorb UV light, chemical derivatization or other methods are required for detection.
2. Quantitative analysis of proteins:
The tryptophan and tyrosine residues in proteins can also absorb UV light near 280 nm, making UV-Vis spectrophotometry a common method for protein quantification. This method is advantageous because it is rapid and does not require labeling. However, the accuracy of this method is affected by the type of protein, as the content of tryptophan and tyrosine may vary significantly between different proteins.
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