What methods are available to eliminate plasma protein interference? What are their characteristics?
Methods to eliminate plasma protein interference mainly include protein precipitation, solid-phase extraction, and immunoaffinity clearance. Each of these methods has distinct characteristics, primarily reflected in the process, efficiency, cost, and applicable sample types.
1. Protein Precipitation:
This is a commonly used method that involves adding organic solvents, acids, or other specific precipitants to precipitate proteins in plasma. Its characteristics include simplicity, speed, and no need for special equipment. However, it may precipitate some small molecules and is not specific enough to completely eliminate all proteins.
2. Solid-Phase Extraction:
This method uses solid-phase media to adsorb target molecules, thereby isolating desired components from complex biological samples. It is characterized by high selectivity and sensitivity and can be used for purification of complex samples. However, it involves multiple steps and is more costly.
3. Immunoaffinity Clearance:
This method uses specific antibodies to identify and clear specific proteins. It is highly specific and can efficiently remove specific proteins with minimal impact on other small molecules. The downside is that it requires high-quality antibodies and is costly, making it unsuitable for bulk sample processing.
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Absolute Quantification of Blood Proteins
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