Common Methods for Protein Methylation Detection?
Protein methylation is an important post-translational modification process. It involves the transfer of methyl groups (from S-adenosylmethionine) to amino acid residues (primarily arginine or lysine) on proteins by methyltransferases, such as protein arginine methyltransferases and protein lysine methyltransferases, thereby affecting the function and activity of proteins. Detecting the methylation status of proteins is a critical step in biological research and disease diagnosis. The following are some common methods for detecting protein methylation:
1. Western Blot Analysis:
This is a commonly used method to detect the presence and amount of specific proteins in samples. By using specific antibodies against methylation sites, this method can detect the methylation status of proteins.
2. Mass Spectrometry:
Mass spectrometry is a powerful tool capable of identifying the exact sites of protein methylation. By comparing with known mass spectra in computer databases, methylation sites in protein samples can be identified.
3. Immunoprecipitation (IP) and Chromatography Techniques:
Using specific antibodies to bind target proteins, the protein-antibody complexes can be separated from mixtures to enrich methylated proteins. These proteins can be further analyzed using liquid chromatography and gas chromatography.
4. Enzyme-linked Immunosorbent Assay (ELISA):
This is a high-throughput method used to detect the presence and amount of specific antigens, such as methylated proteins. Specific antibodies are used to detect and quantify the degree of methylation.
5. Fluorescence Resonance Energy Transfer (FRET):
By using fluorescently labeled antibodies, FRET can monitor the methylation status of proteins in real-time within living cells.
BiotechPack, A Biopharmaceutical Characterization and Multi-Omics Mass Spectrometry (MS) Services Provider
Related Services:
Quantitative Methylation Proteomics Research
Post-translational Modification Proteomics Analysis
Histone Post-translational Modification Analysis
Quantitative Proteomics Analysis
Label-free Quantitative Proteomics Analysis
Label-based Protein Quantification Techniques - iTRAQ, TMT, SILAC
How to order?
