Protein Labeling Quantification
Protein Labeling Quantification is an experimental technique used to analyze protein expression and regulation. Proteins in the experiment bind to specific labeling molecules, aiming to quantitatively analyze the expression levels of specific proteins or multiple proteins in different samples.
The key steps of protein labeling quantification usually include:
1. Protein Extraction:
First, extract proteins from the biological samples being studied (such as cells, tissues, or body fluids).
2. Protein Labeling:
Combine proteins with chemical or isotopic labels. These labels can be stable isotope labeling (such as SILAC), isotopically labeled amino acids (such as iTRAQ), or other specific chemical markers. These labels allow researchers to distinguish and quantify the same proteins in different samples during subsequent analysis.
Figure 1. Protein quantification using SILAC (Esthelle et al., 2019)
3. Protein Separation and Identification:
Separate proteins using techniques such as liquid chromatography (LC) or two-dimensional gel electrophoresis (2D-PAGE). Subsequently, mass spectrometry (MS) is typically used to identify and quantify the labeled proteins.
4. Data Analysis:
Use bioinformatics tools to analyze the acquired data and compare the differences in protein expression under different sample or treatment conditions.
Figure 2. Comparison of SILAC and Dimethyl labeling methods
Protein labeling quantification services have wide applications in biomedical research, drug development, disease diagnosis, and other fields, particularly in studying how proteins are expressed and regulated under different conditions. By quantitatively analyzing protein expression, researchers can better understand the mechanisms of diseases, discover potential biomarkers, or assess the effects of drugs.
BiotechPack, A Biopharmaceutical Characterization and Multi-Omics Mass Spectrometry (MS) Services Provider
Related Services:
Quantitative Proteomics Analysis
Label-Free Quantitative Proteomics Analysis
Label-Based Protein Quantification Techniques - iTRAQ, TMT, SILAC
Quantitative Proteomics Analysis Based on SILAC/Dimethyl Labeling
How to order?
