NMR Peptide Structure Analysis
1. Data Collection
Acquisition of NMR Spectra: Utilizing a nuclear magnetic resonance instrument to collect spectra of peptide samples. One-dimensional and two-dimensional spectra can provide information on chemical shifts and associated coupling constants for peptides.
2. Assignment
NMR Spectra Assignment: Based on the spectra, assign chemical shifts to the atoms of each amino acid residue. Key methods include Nuclear Overhauser Effect (NOE) related spectra and Correlation Spectroscopy (COSY).
3. Calculation and Modeling
Structure Calculation and Analysis: Utilizing the experimental data above, calculate and model the peptide structure using relevant software. Through comparative analysis, the three-dimensional structural characteristics of peptides can be understood and revealed.
NMR (Nuclear Magnetic Resonance) plays a crucial role in studying the structure of macromolecules such as peptides and proteins. It provides atomic-level structural information, which is significant for understanding the structure and function of large molecules like peptides and proteins.
BiotechPack, A Biopharmaceutical Characterization and Multi-Omics Mass Spectrometry (MS) Services Provider
Related Services:
Peptide Structure Determination
Protein Structure Identification
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