Peptide Sequencing

A peptide is a chain of amino acids linked by covalent peptide bonds, which can be divided into oligopeptides (containing 2-20 amino acids) and polypeptides (containing 20-50 amino acids). Peptides often play important roles in organisms in the form of hormones, growth factors, neurotransmitters, etc. The strong efficacy and good tolerance of peptide drugs make them a hot topic in pharmaceutical research.

Peptide sequencing refers to the detection and analysis of the types of amino acids that make up a peptide and their arrangement order. The sequence of a peptide is mainly determined by mass spectrometry-based database searches and de novo sequencing methods. Peptide sequencing is a necessary means to verify whether a known peptide is expressed and to analyze the theoretical amino acid sequence of unknown peptides. The development of peptide sequencing has promoted research on peptide drugs, the discovery of new peptides, and the development of multifunctional peptides.

Biotech company Biotech-Pack employs nano LC-MS/MS combined with Shimadzu's Edman degradation sequencing system to analyze protein sequences, providing services including amino acid composition analysis, N-terminal sequencing, C-terminal sequencing, and full sequence analysis. For proteins with unknown theoretical sequences, we offermass spectrometry-basedproteomic de novo sequencingservices to analyze protein sequences.

Biotech-Pack uses Thermo's latest Obitrap Fusion Lumos mass spectrometer combined with Nano-LC technology to providepeptide sequencingservices to analyze peptide sequences. The Obitrap Fusion Lumos mass spectrometer is currently the highest in resolution and sensitivity, ensuring the sensitivity of low-abundance peptide fragment identification. During peptide fragmentation, a combination of HCD and ETD modes is used to ensure the integrity of peptide fragments. After obtaining the raw mass spectrometry data, for peptides with fewer than 30 amino acids, we use database or de novo sequencing methods to interpret the data. For peptides with more than 30 amino acids, we generally use de novo sequencing to infer peptide sequences.

Related services:

• Mass spectrometry-based protein sequencing analysis
• Peptide de novo sequencing
• Sequence analysis
• Protein sequencing
• Antibody sequencing
• Protein N/C-terminal sequencing
• Complete protein sequencing
• Biopharmaceutical N/C-terminal sequencing
• Protein de novo sequencing
• Peptide sequencing
• De novo protein sequencing and mutation analysis
• Full-length antibody protein sequence analysis
• De novo sequencing