Acylation modification is a type of post-translational modification of proteins. Important cellular metabolites, acyl-CoA compounds, act as donors directly participating in protein acylation modification in organisms, thereby regulating various important biological processes such as epigenetics, energy metabolism, protein transport, and interactions. Protein acylation modification is currently one of the hotspots in life science research. Among protein acylation modifications, acetylation was the first to be discovered and studied extensively. In recent years, other types of acylation modifications such as propionylation, malonylation, succinylation, crotonylation, and others have been discovered. Quantitative proteomics studies of acylation modifications can provide deep insights into the close relationship between changes in protein acylation modifications and life activities by comparing the levels of acylation modifications in biological samples under different physiological or pathological states. This is of great significance for revealing the mechanisms of life activities, screening clinical biomarkers for diseases, identifying drug targets, and more.
In quantitative proteomics analysis of acylation, protein samples are first digested into peptide mixtures. Then, liquid chromatography is used to separate the components of the digested peptide mixtures to reduce sample complexity. Some types of acylation-modified peptides can be specifically enriched using high-quality acylation modification antibodies or kits, and finally analyzed quantitatively through LC-MS/MS.
Biotech company Biotree utilizes the Thermo Fisher Orbitrap Fusion Lumos mass spectrometry platform combined with nanoLC-MS/MS nanoscale chromatography to provide a technical package for acylation quantitative proteomics analysis services. You only need to inform us of your experimental objectives and send your samples to us. We will take care of all subsequent project matters, including protein extraction, protein digestion, acylation peptide enrichment, peptide separation, mass spectrometry analysis, raw mass spectrometry data analysis, and bioinformatics analysis.
Quantitative Proteomics Study of Acylation
About the Samples
1. If you are providing tissue samples, please send them to us on dry ice.
2. If you are providing protein samples, you can use common tissue or cell lysates for protein extraction.
3. Sample transportation: Please use sufficient dry ice for transportation and choose a faster mailing method to minimize the possibility of sample degradation during transportation.
4. Before the formal experiment, we will test the samples you provide. Once they pass the test, the formal experiment will commence.
Bilingual Project Report in Chinese/English
In the technical report, Biotree will provide you with a detailed bilingual version in Chinese and English. The report includes:
1. Experimental steps (in Chinese and English)
2. Relevant mass spectrometry parameters (in Chinese and English)
3. Detailed information of identified acylation sites
4. Mass spectrometry images
5. Raw data
One-Stop Service for Quantitative Proteomics of Acylation
You only need to place an order and send the samples
Biotree's one-stop service includes: sample processing, machine analysis, data analysis, and project report
