What objects is isotope labeling applicable to?
There are many types of isotope labeling methods, such as SILAC (Stable Isotope Labeling by Amino acids in Cell culture), iTRAQ (Isobaric Tags for Relative and Absolute Quantitation), and TMT (Tandem Mass Tags). These methods use stable isotope labeling to accurately distinguish and quantify proteins under different experimental conditions in mass spectrometry analysis. Their applicable research subjects include but are not limited to:
1. Cells:
Isotope labeling methods can be used to study protein expression, modification, degradation, and interaction within cells. Proteins can be extracted from cells under different conditions, such as cells subjected to specific stimuli or treatments.
2. Tissues:
Tissue samples from plants, animals, or other organisms can also be used for quantitative proteomics using isotope labeling methods. This helps in studying tissue-specific protein expression and function.
3. Biological Fluids:
Proteins in biological fluids such as blood, urine, and saliva can be quantitatively analyzed using isotope labeling methods. This is crucial for discovering disease-related biomarkers and key points.
4. Microbial Samples:
Proteins from microorganisms such as bacteria, fungi, and viruses can be quantitatively analyzed using isotope labeling methods. This aids in studying microbial growth, metabolism, and interactions with host cells.
5. Drug Treatment and Disease Models:
By comparing proteomic changes before and after drug treatment or between disease models and normal controls, researchers can study drug mechanisms and disease-related protein changes.
Isotope labeling methods are powerful and versatile in quantitative proteomics and can be widely applied in various research fields of biology, biomedicine, and biotechnology. Through isotope labeling and quantitative analysis of different samples, researchers can gain in-depth understanding of the biological functions of proteins, regulatory mechanisms, and their roles in disease development.
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Related Services:
Quantitative Proteomics Analysis
Tag-based Protein Quantification Techniques - iTRAQ, TMT, SILAC
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