What mass spectrometry methods are used for peptide identification
Peptide identification can be performed through peptide fingerprinting or tandem mass spectrometry. The method involves enzymatically cleaving peptide segments into small peptide mixtures using proteases, followed by mass spectrometry analysis to obtain the amino acid sequences of the small peptide segments for identification. Peptide identification requires two levels of mass spectrometry analysis. The peptide mixture is subjected to primary mass spectrometry to select high-abundance peptide molecules for secondary mass spectrometry. In secondary mass spectrometry, peptide segments collide with each other causing amino acid bond breakage. The resulting peptide fragment ion signals are collected by the detector and a secondary mass spectrum based on mass-to-charge ratio (m/z) is outputted. By interpreting the spectrum, the amino acid sequence of the peptide segment can be analyzed and identified.
BGI-PT offers peptide mass spectrometry identification services using Thermo Fisher's Orbitrap Fusion Lumos mass spectrometry platform combined with nanoLC-MS/MS nanoliter chromatography technology. Peptide mass spectrometry identification service. This service enables the detection of peptide sequence information to obtain peptide sequences or post-translational modification information. You only need to inform us of your experimental purpose and send your samples, and we will take care of all subsequent project matters, including cell culture, protein extraction, protein purification, proteolytic digestion, peptide separation, mass spectrometry analysis, raw mass spectrometry data analysis, and bioinformatics analysis.
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