Methylation Analysis Method

Protein methylation is a common post-translational modification in both eukaryotes and prokaryotes, often occurring on histones and non-histone proteins such as transcriptional regulators, cell signaling molecules, and tumor suppressor proteins. The main tasks of methylation analysis are to identify whether proteins are methylated, the amino acid sites of methylation, and the levels of methylation.

Currently, the main methods for protein methylation analysis are high-performance liquid chromatography-tandem mass spectrometry and antibody methods. Mass spectrometry, with its advantages of high throughput, high precision, and high accuracy, has become an important tool in current protein methylation analysis. The general analysis process involves hydrolyzing the protein to be tested, digesting it into small peptide fragments, enriching the methylated peptides, detecting the methylated peptides separated by high-performance liquid chromatography using tandem mass spectrometry, and finally combining mass spectrometry data with bioinformatics analysis to interpret information such as methylation sites.

Biotechnology company Biotech Pack uses Thermo Fisher's Orbitrap Fusion Lumos mass spectrometry platform combined with nanoLC-MS/MS nanoscale chromatography to providemethylation quantitative proteomics analysistechnical services. You only need to tell us your experimental objectives and send samples. Biotech Pack provides a one-stop service including protein extraction, proteolysis, methylated peptide enrichment, peptide separation, mass spectrometry analysis, mass spectrometry raw data analysis, and bioinformatics analysis. Free consultation is welcome.

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