Protein Mutation Analysis

Protein mutation refers tothe alteration inamino acidstypes orsequencesresulting inchanges,which may furtherlead to changes in the structure and function of proteins.occur.Protein mutations can occur naturally or be induced artificially..Protein mutation analysis primarily involves comparing the differences between normal and mutated proteins to reveal the impact of mutations on protein structure, stability, function, and interactions with other molecules. In biotechnology and drug design, mutation analysis helps in understanding molecular mechanisms of proteins, optimizing protein performance, predicting the biological impact of mutations, and providing a basis for biotechnological and pharmaceutical innovations, thereby improving production efficiency of bioproducts and the specificity of medical treatments.

Biotech company BTP, leveraging the latest Thermo Obitrap Exploris 240 mass spectrometer combined with Nano-LC nanochromatography technology, offers mass spectrometry-based one-stop protein mutation services. These services include high-resolution mass spectrometry analysis of protein amino acid sequences to reveal whether mutations have occurred, mutation sites, and pre- and post-mutation amino acid sequences, supporting research in protein structure and function, gene expression regulation, disease mechanisms, and drug development.

OurAdvantages

1. Advanced Mass Spectrometry Technology:

We utilize the most advanced high-resolution mass spectrometers, including the Q-Exactive and Orbitrap series,to ensure data accuracy and reliability. These instruments can capture extensive protein feature information, providing high-resolution protein mass spectra.

2. Extensive Databases and Algorithms:

We employ various protein sequence databases such as UniProt and Swiss-Prot, and advanced search algorithms like SEQUEST and Mascot to achieve highly accurate protein identification and mutation analysis.

3.High-throughput Data Analysis Platform::

We have built a robust data analysis platform using the latest bioinformatics tools and algorithms to enable protein identification, quantification, functional annotation, enrichment analysis, and more.

4.Rigorous Quality Control::

We adhere to strict quality control processes from sample handling to data analysis to ensure the high accuracy and reproducibility of our services.

5.Customized Services::

We offer tailored experimental designs and data analysis plans to meet specific research objectives based on different client needs.

Main Applications of Protein Mutation Analysis

1. Discovery of New Proteins:

Used to design new proteins, enhance specific functions, or alter structures to meet specific application needs.

2. Protein Structure and Function Research:

Comparing protein sequence and structural differences between different species or individuals,candevelop computational methods to predict protein structure and function, further understanding the formation and evolutionary mechanisms of biological diversity.

3.Molecular Evolution:

Tracing the evolutionary history of organisms,canunderstand evolutionary relationships and functional evolution among different species.

4.DrugDevelopment::

Researching the interactions between proteins and drugs to develop targeted drugs for specific targets.

5.Disease Diagnosis and Treatment:

Can be used to identify protein mutations associated with certain diseases, thereby designing relevant treatment methods.

About Samples

Sample Types	Protein Complexes	Purified Proteins	Cell Lysates	Tissue Samples
Sample Quantity	≥100 ug	≥50 ug	1-5 mg/mL	1-5 mg/mL

*Please avoid using reagents such as detergents, salts, and polyethylene glycol that may interfere with mass spectrometry analysis.

Bilingual Project Report

In the technical report, BTP provides you with a detailed bilingual technical report in both Chinese and English, which includes:

1. Experimental Steps (Chinese & English)
2. Relevant Mass Spectrometry Parameters (Chinese & English)
3. ProteinSequence andMutationSitesDetailed Information
4. Mass Spectrometry Images
5. Raw Data

One-stop Protein Mutation Analysis Service

Simply place an order and send the samples
BTP's one-stop service includes: Sample Processing - Instrumental Analysis - Data Analysis - Project Report

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