Bottom-up and Top-down Mass Spectrometry Analysis Peptides

The main techniques for proteomics research based on biological mass spectrometry include the Bottom-up and Top-down strategies. The Bottom-up strategy involves digesting whole proteins or polypeptides into small peptide fragments for mass spectrometry, which is a more traditional mass spectrometry technique. The Top-down strategy, on the other hand, directly analyzes entire proteins or polypeptides, preserving the original structure, relevant modifications, and unstable structures to the greatest extent, allowing for the capture of more accurate, richer, and more complete molecular biological information of proteins or polypeptides.

Biotech company Thermo's latest Obitrap Fusion Lumos mass spectrometer combined with nanoLC-MS/MS nanoscale chromatography provides both Top-down and Bottom-uppeptide mass spectrometry identificationservice packages, including peptide sequencing, PTMs characterization, and peptide structure characterization, and welcomes free consultations.

Related services:
Peptidomics analysis
Peptide biomarker identification
Peptide purity analysis
Peptide sequencing
De novo peptide sequencing
Protein sequencing analysis based on Top-down method
Top-down proteomics
PTMs characterization based on Top-down method
Bottom-up proteomics
Shotgun protein identification
Protein identification
Protein N-terminal sequencing based on Edman degradation
Protein mass spectrometry identification
Proteomics bioinformatics analysis