How to Quantify Proteins using iTRAQ

iTRAQ(isobaric tags for relative and absolute quantitation) isan in vitro peptide labeling protein quantification technique developed by AB Sciex in the United States. iTRAQ contains 10 different isotopic reagent tags, allowing for the identification and quantification of up to 10 protein samples in a single experiment. The general process of iTRAQ protein quantification technology includes labeling peptides derived from the digestion of different sample proteins with isotopic reagent tags, mixing the labeled peptides in equal amounts, and then performing high-precision tandem mass spectrometry analysis on the mixture. Based on primary and secondary mass spectrometry information such as signal peak intensity, quantitative and qualitative analysis of protein samples can be achieved.

iTRAQ technology can achieve both relative and absolute protein quantification. By labeling an internal standard peptide with iTRAQ reagent, absolute quantification of protein or peptide samples can be achieved by comparing data such as the peak intensity or peak area of the report group of the internal standard peptide to those of the test peptide.

Biotech Pack BioTechnology uses the latest Obitrap Fusion Lumos mass spectrometer from Thermo, combined with Nano-LC, to provideiTRAQ quantitative proteomicsanalysisservicestechnologypackage,you only need to tell us your experimental objectives and send us your proteins, and we will be responsible for all subsequent project matters, including protein digestion, peptide labeling, peptide separation, mass spectrometry analysis, raw mass spectrometry data analysis, and bioinformatics analysis.

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