Isotope Labeling Mass Spectrometry Analysis

Isotope-labeled mass spectrometry is a method that introduces isotope labels into specific biomolecules through chemical methods and uses mass spectrometry technology for quantitative and qualitative analysis. This technology has a wide range of applications in proteomics research, especially in differential proteomics and dynamic protein analysis. As biomedical research advances, the demand for quantitative and identification of proteins in complex biological systems continues to increase. Isotope-labeled mass spectrometry achieves high-precision quantitative analysis by introducing known isotope labels into the sample, allowing differentiation between original and labeled molecules during mass spectrometry detection. The core of isotope-labeled mass spectrometry is the introduction of isotope labels, solving common issues of sample complexity and sensitivity in traditional mass spectrometry analysis, particularly when comparing protein expression levels under different biological conditions. It provides not only relative quantification but also absolute quantification, offering a powerful tool for revealing the function and dynamic changes of proteins in biological systems. In disease research, such as cancer, isotope-labeled mass spectrometry can identify and quantify disease-related biomarkers, providing a basis for early diagnosis, classification, and monitoring of treatment effectiveness. Additionally, in drug development, this analysis can be used for drug target identification, study of drug metabolism pathways, and elucidation of drug mechanisms of action.

 

Isotope-labeled mass spectrometry is generally implemented through three main methods: Stable Isotope Labeling by Amino acids in Cell Culture (SILAC), exogenous labeling (ICAT, TMT, iTRAQ, etc.), and isotope-labeled chemical derivatization (such as DIGE). SILAC achieves protein labeling throughout the process by introducing stable isotope-containing amino acids during cell culture, and is a classic method for in vivo isotope labeling. Exogenous labeling technology connects isotope tags directly to proteins or peptides through chemical reactions without the need for cell culture, making it suitable for various sample sources. In chemical derivatization, chemical methods are used to label proteins or peptides, enabling differentiation of the origin and quantity of different samples in mass spectrometry analysis.

 

Biotech Pack offers mass spectrometry analysis services dedicated to providing customers with high-precision and high-sensitivity protein analysis solutions. Our technical team has extensive experience in proteomics research and can design the most suitable experimental scheme and provide detailed result analysis reports based on the customer's research needs. By collaborating with Biotech Pack, you will receive cutting-edge technical support and a professional customer service experience.

 

Biotech Pack -- Characterization of Biological Products, Quality Service Provider for Multi-Omics Mass Spectrometry Detection

 

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