Can liquid chromatography be used to determine the metabolism of peptides in serum?
Liquid chromatography can be used for peptide separation, but using liquid chromatography alone is usually insufficient to effectively determine the metabolic profile of peptides in serum. This is because peptides in serum are often present at low concentrations and their metabolites are mixed with other complex biomolecules (such as proteins, lipids, etc.), making the detection sensitivity of liquid chromatography inadequate to effectively distinguish these molecules.
To achieve more accurate measurement, liquid chromatography is generally combined with mass spectrometry (LC-MS). The high sensitivity and selectivity of mass spectrometry allow for the detection of the molecular weight and metabolites of peptides. The signal detection of mass spectrometry complements the separation function of liquid chromatography, ensuring precise quantification and identification of peptides.
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